Journal: Cancers

Article Title: Targeted Disruption of E6/p53 Binding Exerts Broad Activity and Synergism with Paclitaxel and Topotecan against HPV-Transformed Cancer Cells

doi: 10.3390/cancers14010193

Figure Lengend Snippet: The cell-cycle arrest induced by Cpd12 occurs in the G1 phase. ( A ) The effects of Cpd12, the inactive small molecule Cpd3, or DMSO, on cell cycle modulation were evaluated by cytofluorimetric analysis of HPV-positive cervical (ME180 and MS751) and head-and-neck (SCC152) cancer cells treated for 24 h and then stained with PI. Bar graphs represent the percentage of cells in the different phases of the cell cycle (G0/G1, M, G2/S) as the mean of three independent experiments. ( B ) Cell-cycle distributions of HPV-positive cervical (ME180, MS751, HeLa, CaSki, and SiHa) and head-and-neck (SCC152) cancer cells treated with Cpd12 (50 µM) or DMSO for 24 h were determined through immunofluorescence assays. Cells in either the G0, G1, S, G2, or M phase were determined as a function of Ki67 and nuclear staining, and fluorescence intensities were quantified from maximum intensity projections of Z-stack images acquired with a 600× magnification. Total numbers of cells were: n DMSO = 463 and n Cpd12 = 304 for ME180 cells, n DMSO = 303 and n Cpd12 = 316 for MS751 cells, n DMSO = 314 and n Cpd12 = 267 for SCC152 cells, n DMSO = 303 and n Cpd12 = 270 for HeLa cells, n DMSO = 388 and n Cpd12 = 330 for CaSki cells, and n DMSO = 336 and n Cpd12 = 244 for SiHa cells. Nuclei were stained with DRAQ5. 2N: diploid DNA content, 4N: tetraploid DNA content.

Article Snippet: HPV-positive HeLa (HPV18), CaSki (HPV16), SiHa (HPV16), ME180 (HPV68), MS751 (HPV45), and HPV-negative C33A cervical cancer cells, HPV-positive SCC152 (HPV16) and HPV-negative FaDu head-and-neck cancer cells, and human foreskin fibroblasts (HFF) were all from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Immunofluorescence, Fluorescence

Journal: Cancers

Article Title: Targeted Disruption of E6/p53 Binding Exerts Broad Activity and Synergism with Paclitaxel and Topotecan against HPV-Transformed Cancer Cells

doi: 10.3390/cancers14010193

Figure Lengend Snippet: The Cpd12-induced rescue of p53 affects the migration capacity of HPV-transformed cells. ( A ) Confluent monolayers of HeLa, CaSki, and ME180 cervical cancer cells were scraped up two times and then treated with increasing concentration of Cpd12 (10, 25, and 50 µM) in low serum medium. The wound closure was monitored before treatment and after 12, 24, and 48 h, and representative images were taken using a bright-field inverted microscope (100× magnification). ( B ) HeLa cells were treated with Cpd12 (50 μM) for 24 h. Then, cell migration was evaluated by transwell assays after an incubation of other 24 h in Cpd12-free medium. Representative images of migrated cells fixed and colored with crystal violet were taken using a bright-field inverted microscope (100× magnification). In both panels, cells treated with DMSO or Cpd3 (50 μM) were included as negative controls.

Article Snippet: HPV-positive HeLa (HPV18), CaSki (HPV16), SiHa (HPV16), ME180 (HPV68), MS751 (HPV45), and HPV-negative C33A cervical cancer cells, HPV-positive SCC152 (HPV16) and HPV-negative FaDu head-and-neck cancer cells, and human foreskin fibroblasts (HFF) were all from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Transformation Assay, Concentration Assay, Inverted Microscopy, Incubation

Journal: Cancers

Article Title: Targeted Disruption of E6/p53 Binding Exerts Broad Activity and Synergism with Paclitaxel and Topotecan against HPV-Transformed Cancer Cells

doi: 10.3390/cancers14010193

Figure Lengend Snippet: Analysis of the effect of the drug combination on HPV-positive cervical and head-and-neck cancer cells viability.

Article Snippet: HPV-positive HeLa (HPV18), CaSki (HPV16), SiHa (HPV16), ME180 (HPV68), MS751 (HPV45), and HPV-negative C33A cervical cancer cells, HPV-positive SCC152 (HPV16) and HPV-negative FaDu head-and-neck cancer cells, and human foreskin fibroblasts (HFF) were all from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: